TitleExtraction of sardine myoglobin and its effect on gelation properties of Pacific whiting surimi.
Publication TypeJournal Article
Year of Publication2007
AuthorsPark, JD, Park, JW
JournalJ Food Sci
Date Published2007 May
KeywordsAmmonium Sulfate, Analysis of Variance, Animals, Blood Proteins, Calorimetry, Differential Scanning, Cattle, Electrophoresis, Polyacrylamide Gel, Ethanol, Fish Products, Fish Proteins, Gadiformes, Gelatin, Myoglobin, Pacific Ocean, Pigments, Biological, Rheology, Temperature

Myoglobin (Mb) was extracted from Pacific sardine and added to Pacific whiting surimi to measure its effects on protein gelation. The purity of Mb extract was determined by SDS-PAGE. Mb extract using ethanol showed higher purity than Mb extract using ammonium sulfate. The addition of 0.2% Mb significantly reduced breaking force. However, a synergistic effect of 1.0% Mb was observed with 1.0% beef plasma protein (BPP) to increase surimi gel strength. The highest storage modulus of gels was observed at 1.0% Mb addition, which corresponded with the nonfracture gel and also supported a synergistic interaction between 1.0% Mb and 1.0% BPP. Differential scanning calorimetry showed Mb addition might have affected myosin denaturation and aggregation.

Alternate JournalJ. Food Sci.
PubMed ID17995761